Plasmid_Backbone

Part:BBa_J428388

Designed by: Marcos Valenzuela-Ortega   Group: 2021_Engineering_Committee   (2022-06-16)


pJUMP19-min2 Level 0 Minimal cargo vector.

Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors.

Please view and default to the original genbank files from the Supplementary Data in https://doi.org/10.1093/synbio/ysab003 as the Registry sequence is in draft.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3026
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3032
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3026
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3026
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3026
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3041
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 853
    Illegal NgoMIV site found at 982
    Illegal NgoMIV site found at 1476
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2274


[edit]
Categories
Parameters
None