Regulatory

Part:BBa_J23100:Design

Designed by: John Anderson   Group: iGEM2006_Berkeley   (2006-08-04)

constitutive promoter family member

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

Fwd: GCTTCTAGAGATACATGAACATGCAATACttgacggctagctcagtcctaggtacagtgctagc

Rv: CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg

Source

Isolated from library of promoters

References