Plasmid
pSB1AC3F

Part:BBa_J18901

Designed by: Raik Gruenberg   Group: Affiliates   (2008-08-11)


pSB1AC3F construction plasmid for protein fusion BioBricks

pSB1AC3F is identical to pSB1AC3 but uses the "Freiburg" extension of the standard BioBrick prefix and suffix. This Freiburg extension adds a new pair of inner restriction enzymes -- NgoMIV (prefix) and AgeI (suffix) -- which allows the in-frame fusion of protein parts. See BB formats.

pSB1AC3F is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.

The given sequence starts with the Freiburg BB suffix and ends with the Freiburg BB prefix. The complete vector sequence is therefore obtained from: part + vector

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3043
    Illegal suffix found in sequence at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3043
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 3049
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3043
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1936
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3043
    Illegal suffix found in sequence at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3043
    Illegal suffix found in sequence at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2082
    Illegal EcoRI site found at 3043
    Illegal XbaI site found at 3058
    Illegal SpeI site found at 11
    Illegal PstI site found at 25


[edit]
Categories
//plasmidbackbone/proteinfusion
Parameters
None