Plasmid_Backbone
V0200

Part:BBa_J176121

Designed by: Brady Laughlin   Group: Haynes_Lab   (2011-10-18)

V0200

FRT-marked mammalian transfection vector (from Invitrogen pcDNA5 FRT/TO); no promoter; amp, hygro


Usage and Biology

  • Used to create a stable insert into a mammalian chromosome
  • Must be co-transfected with a plasmid expressing Flp recombinase
  • Host cells must carry a FRT target site


Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1238
    Illegal XbaI site found at 970
    Illegal SpeI site found at 329
    Illegal PstI site found at 1328
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1238
    Illegal SpeI site found at 329
    Illegal PstI site found at 1328
    Illegal NotI site found at 309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1238
    Illegal BglII site found at 47
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1238
    Illegal XbaI site found at 970
    Illegal SpeI site found at 329
    Illegal PstI site found at 1328
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1238
    Illegal XbaI site found at 970
    Illegal SpeI site found at 329
    Illegal PstI site found at 1328
    Illegal NgoMIV site found at 831
    Illegal NgoMIV site found at 2093
    Illegal AgeI site found at 396
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 378
    Illegal BsaI.rc site found at 3621
    Illegal EcoRI site found at 1238
    Illegal XbaI site found at 970
    Illegal SpeI site found at 329
    Illegal PstI site found at 1328


[edit]
Categories
Parameters
n/aV0200