Primer

Part:BBa_J133701

Designed by: Norman Wang   Group: Presting Lab   (2011-06-24)


primers tail extension SP for BioBricking

Extend your cloning primers with BioBrick Prefix cutting sites (SpeI-NotI-PstI) by concatenating this sequence on the 3' end. Sequence is designed to minimize primer homodimers with itself, and heterodimer with BBa_J133700.

Make sure to order the primer in the correct 5'->3' orientation and the correct strand for the reverse primer!

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None