Composite

Part:BBa_J120030

Designed by: Xiaoyang Feng, Orijit Kar, Stephen Cain, Sanjana Gurrala, Sahil Sharma   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-04)

Composite part with Yeast promoter,CH1 domain of human Ab, ADAM17 cleavage sequence & Phoratoxin.

This composite part contains a Yeast promoter part BBa_J63006 along with the CH1 domain of human antibody BBa_J120020 , Adam17 cleavage sequence BBa_J120023, a phoratoxin BBa_J120022 and a terminator BBa_B0011. This part is built for experimental purposes and will be tested in yeast to check for the functionality of the phoratoxin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None