Coding

Part:BBa_J120001

Designed by: Corinthia Black, Sheree Harper, Srikanth Sistla, Anna Wieman   Group: N Iowa-840:153g - Recombinant DNA Techniques - F10   (2012-04-10)

DFFb (CAD)-coding sequence for H. sapien DNA fragmentation factor beta

DFFb (DNA fragmentation factor beta polypeptide) a.k.a CAD (caspase activated DNase) is activated by cleavage from its inhibitor by Caspase 3. Once free, CAD forms homo-dimers with other free CAD molecules leaving at crevice at the C3 domain which aids in the function of DNA fragmentation by essentially forming two blades in a pair of scissors (Woo et al., 2004) This is a key step in apoptosis. This sequence was originally obtained from NCBI (accession # NM_004402.2, but we modified the sequence to prevent cleavage by restriction enzymes at internal restriction sites. This was done by replacing the third base at each of these codons.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 488
    Illegal BamHI site found at 749
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 397


[edit]
Categories
Parameters
None