RBS

Part:BBa_J119393:Design

Designed by: Anthony J. Eckdahl   Group: Eckdahl_Lab   (2016-04-29)


Collection of Bicistronic RBSs from N8 rClone Red Library


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The oligos needed to include a 5' sticky end of CGAC and a 3'sticky end of CCGC to ligate into rClone Red with BsaI Golden Gate Assembly.


Source

Synthetic oligos cloned into rClone Red (BBa_J119384).

References