Plasmid_Backbone
pSB1A8

Part:BBa_J119043:Design

Designed by: Todd Eckdahl and A. Malcolm Campbell   Group: Eckdahl_Lab   (2012-01-04)

pSB1A8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2207
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2207
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2207
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2222
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

BsaI site removed from amplicillin resistance gene.
Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.

Source

De novo DNA synthesis.

References