Part:BBa_J119006

This is RFP-2, the second half of the RFP Gene Template to be used in conjunction with RFP-1 (J119005) to act as a node for solving Hamiltonian Path Problems (HPP). When successfully re-assembled will report with a red fluorescence that may be visible under normal light conditions. Can be used with other split genes to solve more complex HPP problems.

RFP_2_For (36mer)

5’ GCAT GAATTC GGTCTC A CTGC AAGACGGTGAGTTCA 3’

(4, EcoRI , BsaI, a , Gene1234 , 15mer of RFP)

RFP_2_Rev (75mer)

5’ATGC CTGCAG CGTCTC A CCAC GCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAA TTGC TTATTAAGCACCGGT 3’

(4nt, PstI , BsmBI , a , Halfedge1234RC , J23100 Reverse Complement , 4nt , 15mer of RFP Rev)