RBS

Part:BBa_J100471:Design

Designed by: Maria Mavrodieva   Group: Campbell_M_Lab   (2018-11-08)


RBS non-Watson Crick base pairing (A-A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the design for part J100454, which was an RBS with non-Watson Crick base pairing (A-C). This base pairing has two atypical hydrogen bonds. This part is testing the affects of only one atypical hydrogen bond on the non-Watson Crick base pairings (A-A).


Source

rClone Red Facilitates Bacterial Gene Expression Research by Undergraduates in the Teaching Laboratory A. Malcolm Campbell 1,2, Todd T. Ekdahl

References