RBS

Part:BBa_J100466:Design

Designed by: Bianca Nolde-Lopez   Group: Campbell_M_Lab   (2018-11-08)


E.coli Plasmid Ribosomal Binding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We are combating the disruptive nature of NWC base pairing next to NWC by adding Watson and Crick base pairing between each NWC. Lastly this part has no hair pinning within the RBS because we added a buffer of cytosine between the DNA sequence upstream and the start codon.


Source

E.coli plasmid

References