RBS

Part:BBa_J100457:Design

Designed by: Brooke Whitcomb   Group: Campbell_M_Lab   (2018-10-23)


RBS for 16s rClone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want the DNA control element to ligate into the E. Coli plasmid rClone Red, so we want to use strong hydrogen bonds like in our first RBS BBa_J100440. We have not changed our RBS sequence from BBa_J100440, but we have added nucleotides before RBS to affect folding. We want to make our sequence fold in a manner that allows ribosome recognition, causing the sequence to be translated often.



Source

no source information for this part.

References