RBS

Part:BBa_J100112:Design

Designed by: Jessica Gronniger   Group: Campbell_M_Lab   (2013-06-29)


BD24 C.dog cloned into J100110


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No internal BsaI sites. The 5' end of Junction C from part BBa_J119301 was changed from TTTC to GTTC. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100112. The 5' end (future Bsa I sticky end) for Junctions D also need to be changed. It will be changed with the next round of iPCR. The 5' end of Junction D will be changed from TTAA to CGGA.

Source

All parts taken from Registry.

References