Generator

Part:BBa_I766382

Designed by: Michael Chen   Group: iGEM07_UCSF   (2007-10-25)

OspF-RR under inducible promotor

OspF-RR under inducible promotor

Usage and Biology

OspF is a bacterial phosphothreonine lyase, apparently specific for MAPKs. It catalyzes the removal of the phosphorylated threonine side chain present in the MAPK activation loop, therefore inactivating the kinase in an irreversible manner. Here, OspF is N-terminally fused to mCherry, and C-terminally to the synthetic leucine zipper RR; being expressed under control of a Fig1 promoter (inducible by mating pathway activation).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2104
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2104
    Illegal NotI site found at 2663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1576
    Illegal BglII site found at 1660
    Illegal BamHI site found at 2646
    Illegal XhoI site found at 1744
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2104
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2104
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


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