Generator
Part:BBa_I766381
Designed by: Michael Chen Group: iGEM07_UCSF (2007-10-25)
OspF-RR under medium constituitive promotor
OspF-RR under medium constituitive promotor
Usage and Biology
OspF is a bacterial phosphothreonine lyase, apparently specific for MAPKs. It catalyzes the removal of the phosphorylated threonine side chain present in the MAPK activation loop, therefore inactivating the kinase in an irreversible manner. Here, OspF is N-terminally fused to mCherry, and C-terminally to the synthetic leucine zipper RR; being expressed under control of a Cyc1 promoter (constitutive medium level expression).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1348
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1348
Illegal NotI site found at 1907 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 820
Illegal BglII site found at 904
Illegal BamHI site found at 1890
Illegal XhoI site found at 988 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1348
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1348
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |