Generator
Part:BBa_I766238
Designed by: Nili Sommovilla Group: iGEM07_UCSF (2007-10-25)
WMTM1-EE under strong inducible promoter
Usage and Biology
Construct is under control of pAdh, a strong constitutive promoter.
EE is one half of the EE-RR leucine zipper interaction, which has a dissociation constant in the nanomolar range.
WMTM1 stands for Worm (C. Elegans) Myotubularin(MTM) related protein 1. WMTM1 binds Phosphatidylinositol 3-Phosphate (PI[3]P) and Phospatidylinosotol 3,5-Phosphate (PI[3,5]P) as substrates and dephosphorylates them at the 3-Phosphate position. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 195
Illegal EcoRI site found at 1568
Illegal EcoRI site found at 1714 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 195
Illegal EcoRI site found at 1568
Illegal EcoRI site found at 1714
Illegal NheI site found at 2009 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 195
Illegal EcoRI site found at 1568
Illegal EcoRI site found at 1714
Illegal BglII site found at 1100
Illegal BamHI site found at 2998
Illegal XhoI site found at 1012 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 195
Illegal EcoRI site found at 1568
Illegal EcoRI site found at 1714 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 195
Illegal EcoRI site found at 1568
Illegal EcoRI site found at 1714
Illegal AgeI site found at 619 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |