Generator

Part:BBa_I766238

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-25)

WMTM1-EE under strong inducible promoter

Usage and Biology

Construct is under control of pAdh, a strong constitutive promoter.

EE is one half of the EE-RR leucine zipper interaction, which has a dissociation constant in the nanomolar range.

WMTM1 stands for Worm (C. Elegans) Myotubularin(MTM) related protein 1. WMTM1 binds Phosphatidylinositol 3-Phosphate (PI[3]P) and Phospatidylinosotol 3,5-Phosphate (PI[3,5]P) as substrates and dephosphorylates them at the 3-Phosphate position. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1568
    Illegal EcoRI site found at 1714
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1568
    Illegal EcoRI site found at 1714
    Illegal NheI site found at 2009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1568
    Illegal EcoRI site found at 1714
    Illegal BglII site found at 1100
    Illegal BamHI site found at 2998
    Illegal XhoI site found at 1012
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1568
    Illegal EcoRI site found at 1714
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 195
    Illegal EcoRI site found at 1568
    Illegal EcoRI site found at 1714
    Illegal AgeI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


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