Intermediate
Part:BBa_I759001
Designed by: Josh Michener Group: iGEM07_Caltech (2007-06-27)
pTet-spacer-N
A pTet promoter (can be repressed by tet Repressor, and transcriptional activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-N complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. This construct is an intermediate, as the terminator sequence for the transcript has not yet been cloned it. The finished construct can produce an aTc and cis-dependent level of N protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |