Generator

Part:BBa_I746914

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-10-01)

pbad promoter driving 6-his tagged superfolder GFP

This part is used for purification of superfolder GFP (for source and other information about this new GFP variant see its part description: I746916 and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP) via its 6-his tag. It is driven by the arabinose - sensitive pbad promoter. Induction was with 1mM arabinose for overnight growth of a 50ml culture for small scale purification the next day.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None