Translational_Unit
rbs+crtIB

Part:BBa_I742161

Designed by: Chris French   Group: iGEM07_Edinburgh   (2007-10-24)

crtI (phytoene dehydrogenase) and crtB (phytoene synthase)

crtIB genes encoding phytoene dehydrogenase and phytoene synthase from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the mutated gene has not been confirmed. This version still contains the two PstI sites. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)


Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
    Illegal NgoMIV site found at 2061
    Illegal NgoMIV site found at 2191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal PstI site found at 500
    Illegal PstI site found at 911


[edit]
Categories
//classic/generator/RC
//function/biosynthesis/isoprenoid
Parameters
None