Composite

Part:BBa_I742140

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-22)


Vanillin Biosynthesis genes

Construct contains all five genes required to synthesise vanillin (the major component of vanilla scent) from tyrosine.

The five genes in this pathway are, Sam5, Sam8, COMT, ech and fcs.

Details of the vanillin biosynthesis pathway may be found on the [http://2007.igem.org/Edinburgh/Yoghurt/Design#Vanilla_Flavour_Production Edinburgh Yoghurt Wiki]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2374
    Illegal BamHI site found at 2776
    Illegal BamHI site found at 6212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1436
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 45
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 968
    Illegal NgoMIV site found at 1370
    Illegal NgoMIV site found at 1374
    Illegal NgoMIV site found at 2117
    Illegal AgeI site found at 2064
    Illegal AgeI site found at 2233
    Illegal AgeI site found at 4800
    Illegal AgeI site found at 5337
    Illegal AgeI site found at 5798
    Illegal AgeI site found at 6240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1668
    Illegal BsaI site found at 1934
    Illegal BsaI.rc site found at 569
    Illegal BsaI.rc site found at 3717


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Parameters
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