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Applications of BBa_I742130
Glasgow 2014 experience
We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick BBa_K1463520. This can be seen in Fig. 1 below.
It gave a lower fluorescence than our designed reverse RBS BBa_K1463560.
Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.
BBa_I742130 calculated translation initiation rate 495.33
BBa_K1463560 calculated translation initiation rate 12,204.43