Part:BBa_I732958

Ptemplate1 + RBS.ARL4A0604.TERM

Genotype	Character	Name
Top10/pT-TERM	So not express any repressors	CR00
Top10/pT-ARL4A0604	Constitutively express ARL4A0604	CR01
Top10/pT-ARL2A0203	Constitutively express ARL2A0203	CR10
Top10/pTet-ARL4A0604-ARL2A203	Constitutively express ARL4A0604 and ARL2A0203	CR11

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 2209
  Illegal EcoRI site found at 4344
  Illegal XbaI site found at 4509
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4344
  Illegal SpeI site found at 2210
  Illegal PstI site found at 2224
  Illegal NotI site found at 2217
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4344
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 2210
  Illegal EcoRI site found at 4344
  Illegal XbaI site found at 4509
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4344
  Illegal XbaI site found at 4509
  Illegal SpeI site found at 2210
  Illegal PstI site found at 2224
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3383
  Illegal BsaI.rc site found at 4368