Coding

Part:BBa_I712028:Design

Designed by: Marko Bitenc   Group: iGEM07_Ljubljana   (2007-10-21)


CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 708
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the original cherry protein there was a PstI restriction site in the middle of the sequence. That is why mutagenesis of this restiction site was necessary.


Source

From one clone that Gabi gave me - will be further described in the future.

References