ccdB and minimal pUC19 derived high copy origin
BBa_I52002 is the default part cloned into the BioBrick cloning site of the BioBrick base vector BBa_I51020 and by extension all BioBrick standard vectors constructed from BBa_I51020. BBa_I52002 is composed of two parts BBa_P1016 and BBa_I50022. BBa_P1016 is the positive selection marker ccdB used to facilitate assembly of BioBrick parts. BBa_I50022 is a minimal high copy pUC19-derived replication origin. BBa_I50022 enables the base vector to be propagated at high copy for easy plasmid DNA purification.
Usage and Biology
For more information on how to use this BioBrick part, visit the Featured Parts:Cell Death page.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 435
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 323