ccdB and nonfunctional pUC19 derived high copy origin
BBa_I52001 is composed of two parts: BBa_P1016 and BBa_I50020. BBa_P1016 is the positive selection marker ccdB used to facilitate assembly of BioBrick parts. BBa_I50020 is a nonfunctional minimal high copy pUC19-derived replication origin.
Plasmids carrying BBa_I52001 as the default part cloned into the BioBrick cloning site are functional BioBrick vectors. Unlike plasmids carrying BBa_I52002, they will propagate at the copy number of the vector replication origin.
Usage and Biology
For more information on how to use this BioBrick part, visit the Featured Parts:Cell Death page.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 435
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 323