pUC19 ori


Designed by: Reshma Shetty   Group:   (2008-01-05)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I50022

User Reviews



Reshma Shetty

BBa_I50022 is a subpart of the default insert BBa_I52002 in BioBrick standard vectors pSB4A5, pSB4K5, pSB4C5 and pSB3K5 and the BioBrick base vector BBa_I51020. All new BioBrick vectors constructed from the BioBrick base vector BBa_I51020 will also contain the default cloned part BBa_I52002. BBa_I50022 ensures that BioBrick standard vectors and the BioBrick base vector propagate at high copy irrespective of the vector replication origin. Miniprep yields from plasmids containing BBa_I50022 tend to be very high (of order >100 ng/μL from a 5mL overnight culture).