Regulatory
Prm +

Part:BBa_I12007:Design

Designed by: Hans   Group: Antiquity   (2004-07-15)


Modified lambda Prm promoter (OR-3 obliterated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.


Source

Shih and Gussin (1983)

References

  • Shih and Gussin, "Mutations affecting two different steps in transcription initiation at the phage lambda Prm promoter", Proc. Natl. Acad. Sci. USA Vol. 80, pp. 496-500, January 1983