Coding
GFP

Part:BBa_E0040:Design

Designed by: jcbraff   Group: Antiquity   (2004-09-30)

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644

Design Notes

GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect.

Source

This part was derived from the reporter device used by Elowitz's repressilator Elowitz. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers Andersen to build the reporter device. Andersen constructed those GFP variants based on a gfpmut3b containing plasmid that was a gift from R. H. Valdivia Valdivia. The PCR method used in construction by Andersen changed aa residue 2 from serine to arginine with no detectable phenotypic effect in their experiments.

References

<biblio>

  1. Elowitz pmid=10659856
  2. Andersen pmid=9603842
  3. Valdivia pmid=8707053

</biblio>