Terminator

Part:BBa_B0015

Designed by: Reshma Shetty   Group: Antiquity   (2003-07-17)

Terminator.png

biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiency0.984[CC]
0.97[JK]
reversed_version2146
reverse_efficiency0.295[CC]
0.62[JK]

double terminator (B0010-B0012)

  • Double terminator consisting of BBa_B0010 and BBa_B0012.
  • This is the most commonly used terminator. It seems to be reliable.
    Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination.


B0010

B0012

Secondary Structure

The mfold results are annotated with the location of the subparts BBa_B0010 and BBa_B0012 and the BioBrick assembly scar.

Mfold-B0015-1.png


Using primers VR/VF2 to PCR B0010 will result in excess bands.

See a full description of the problem here.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Measurement

[edit]
Categories
//direction/forward
//terminator/double
Parameters
biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiency0.984[CC]
0.97[JK]
reversed_version2146
reverse_efficiency0.295[CC]
0.62[JK]