Terminator

Part:BBa_B0012:Design

Designed by: Reshma Shetty   Group: Antiquity   (2003-01-31)


TE from coliphageT7

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).

Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.



Source

Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 [Genbank V01146 ].

References