|Reshma Shetty, Drew Endy and Tom Knight developed a method and set of parts (available via the Registry) to make the process of building a new BioBrick® plasmid backbone easier. The method and part collection are described in their open-access paper, Engineering BioBrick vectors from BioBrick parts published in the Journal of Biological Engineering.|
How to construct a new BioBrick® plasmid backbone
This plasmid construction approach enables reuse of replication origins and antibiotic resistance markers available from the Registry of Standard Biological Parts. Use of the base vector to construct all BioBrick® standard construction plasmids ensures standardization and uniformity in any resulting BioBrick® vectors.
Entering new BioBrick plasmid backbones into the Registry
Please see the help page on entering new plasmid backbones and their default inserts into the Registry for details.
Construction plasmids built from the BioBrick base vector
Using the approach outlined above, seven new BioBrick construction plasmids were constructed.
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|Building new BioBrick vectors: A complete listing of all BioBrick parts that might help you in building a new BioBrick standard construction plasmid.|