Several parameters are identified to help us understand the advantages and disadvantages associated with using a particular chassis.
|Basic criterion for a chassis||Ability to express Biobricks|
|Temperature dependence||The relationship between expression rate and temperature.|
|Optimum amount of DNA||Amount of DNA required for expression rate to reach a maximum value.|
|Product stability||Measure of the half-life of a given protein (e.g. GFP) synthesized in the chassis.|
|Measure of time from start of reaction to the point when expression rate reaches the maximum value|
Measure of time from start of reaction to the point when protein degradation overrides protein synthesis
|Expression capacity||Measure of the total expression of a chassis for a given DNA construct template. This should take into account the degradation of synthesized protein.|
To gain knowledge about the cell-free chassis, we have selected the following parts available in the Registry to extract the above-mentioned properties.