Help:Assembly standard 21
Researchers at UC Berkeley have developed the BglBrick assembly standard, or Assembly standard 21, based on idempotent assembly with BamHI and BglII restriction enzymes. In a nutshell, most parts look like this:
Prefix Suffix 5' GAATTC atg AGATCT ...part... GGATCC taa CTCGAG 3' EcoRI BglII BamHI * XhoI
Fusing two parts leaves the following scar:
5' [part A] GGATCT [part B] 3' G S
Note, however, that Assembly standard 20 is intended as a minimal physical assembly standard, and only those features needed for interconversion of BglBrick assembly standard plasmids are formally defined. Therefore,
taa spacers are not core definitions of the standard.
See The BioBricks Foundation wiki for a discussion and comparison of different technical standards.
- A Berkeley assembly standard part is a DNA sequence flanked on the 5' end by
GATCTand on the 3' end by
Glacking BglII, BamHI, EcoRI, and XhoI restriction sites
- A Berkeley assembly standard vector is a DNA sequence flanked on its 5' end by
GATCCand on its 3' end by
- A Berkeley assembly standard entry vector has a unique EcoRI site, no BamHI or BglII restriction sites, and at most one XhoI site 5' to the EcoRI site
- A Berkeley assembly standard plasmid is represented as <vector_name>-<part_name> and has the sequence obtained by concatenating the vector and part sequences
- Further definition constraints are "sub-standards" of the Berkeley assembly standard format
- in-frame fusion of protein parts
- benign protein scar
- enzymes selected for efficient cutting
- BglII cannot be heat-inactivated therefore the current 3A standard assembly procedures won't work
- incompatible to original BioBrick assembly standard format
- incompatible to BioFusion format
- Anderson, J Christopher; Dueber, John E; Leguia, Mariana; Wu, Gabriel C; Goler, Jonathan A; Arkin, Adam P; Keasling, Jay D. BBF RFC 21: BglBricks Assembly Standard. BBF RFC 21. 2009.