Frequently Used Parts

The iGEM Registry has a vast collection of parts that have been contributed over the course of our 10 year history. We have more than 20,000 parts that iGEM students (collegiate and high school), graduate students, postdocs, and PIs have contributed to the collection. Some of our parts have been used many, many times. In general, these are the older parts in our collection that have served as the foundation of many projects.

The job of every iGEMer and contributor to the Registry is to make parts users will want to use in coming years. With over 20,000 parts already in our collection, this is not an easy feat. You will need to create great parts for them to become among our most used.

We have many ribosome binding sites in our collection. You may want a strong RBS or one for your organism of choice. However, if you're not sure, you might want one that many other people have used, and will just work in . At the time of writing, our most frequently used part is BBa_B0034. This ribosome binding site, first described by Michael Elowitz in 1999 has been used over 8000 times in the history of the Registry. You can find the number of uses for each part in the part quick reference box at the top of each part page.

The purpose of this page is to showcase the most highly-used parts in our collection so you may see how iGEMers on different teams approach making great parts.

Top 10 Most Used Parts on the Registry

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0034RBS (Elowitz 1999) -- defines RBS efficiency12Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 3713In stock950
BBa_B0015double terminator (B0010-B0012)129Reshma Shetty3210In stock1432
BBa_R0040TetR repressible promoter54 June Rhee, Connie Tao, Ty Thomson, Louis Waldman900In stock1355
BBa_B0030RBS.1 (strong) -- modified from R. Weiss15Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 734In stock1238
BBa_B0010T1 from E. coli rrnB80Randy Rettberg657In stock1064
BBa_R0010promoter (lacI regulated)200 654In stock1524
BBa_B0012TE from coliphageT741Reshma Shetty649In stock904
BBa_B0032RBS.3 (medium) -- derivative of BBa_003013Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 606In stock1095
BBa_E0040 green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212720jcbraff593In stock4040
BBa_R0011Promoter (lacI regulated, lambda pL hybrid)55Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton498In stock1393

Top 10 Most Used Coding Region Parts

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_E0040 green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212720jcbraff593In stock4040
BBa_E1010 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)706Drew Endy360In stock1378
BBa_C0051cI repressor from E. coli phage lambda (+LVA) 775Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross256In stock1310
BBa_C0040tetracycline repressor from transposon Tn10 (+LVA)685June Rhee, Connie Tao, Ty Thomson, Louis Waldman.251In stock1120
BBa_C0012lacI repressor from E. coli (+LVA)1153Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton214In stock1225
BBa_C0062luxR repressor/activator, (no LVA?)781Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr200In stock1350
BBa_C0061autoinducer synthetase for AHL643Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr188In stock1361
BBa_E0030enhanced yellow fluorescent protein derived from A. victoria GFP723Caitlin Conboy and Jennifer Braff128In stock754
BBa_E0020engineered cyan fluorescent protein derived from A. victoria GFP 723Caitlin Conboy and Jennifer Braff79In stock754
BBa_C0079lasR activator from P. aeruginosa PAO1(+LVA)781Alvin Carter Powers (Fighting Darwins)58In stock823

Top 10 Most Used Promoters

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_R0040TetR repressible promoter54 June Rhee, Connie Tao, Ty Thomson, Louis Waldman900In stock1355
BBa_R0010promoter (lacI regulated)200 654In stock1524
BBa_R0011Promoter (lacI regulated, lambda pL hybrid)55Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton498In stock1393
BBa_J23100constitutive promoter family member35John Anderson406In stock1409
BBa_I0500Inducible pBad/araC promoter1210Sri Kosuri343In stock3742
BBa_R0062Promoter (luxR & HSL regulated -- lux pR)55Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr317In stock1773
BBa_J23119constitutive promoter family member35John Anderson259In stock816
BBa_R0051promoter (lambda cI regulated)49Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross232In stock1062
BBa_J23101constitutive promoter family member35John Anderson215In stock894
BBa_I13453Pbad promoter130jkm184In stock3744

Top 10 Most Used Ribosome Binding Sites

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0034RBS (Elowitz 1999) -- defines RBS efficiency12Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 3713In stock950
BBa_B0030RBS.1 (strong) -- modified from R. Weiss15Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 734In stock1238
BBa_B0032RBS.3 (medium) -- derivative of BBa_003013Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 606In stock1095
BBa_B0031RBS.2 (weak) -- derivative of BBa_003014Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.200In stock1094
BBa_Z0262Medium strength T7.2 RBS20Sriram Kosuri117Not in stock886
BBa_Z0261Strong T7.2 RBS20Sriram Kosuri98Not in stock884
BBa_J61100Ribosome Binding Site Family Member12John Anderson95In stock1547
BBa_J176012Kozak18Brady Laughlin56In stock775
BBa_J61101Ribosome Binding Site Family Member12John Anderson48In stock1508
BBa_K1362090strong T7 RBS29Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha41It's complicated 

Top 10 Most Used Terminators

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0015double terminator (B0010-B0012)129Reshma Shetty3210In stock1432
BBa_B0010T1 from E. coli rrnB80Randy Rettberg657In stock1064
BBa_B0012TE from coliphageT741Reshma Shetty649In stock904
BBa_B0014double terminator (B0012-B0011)95Reshma Shetty266In stock998
BBa_B1006Terminator (artificial, large, %T~>90)39Haiyao Huang141In stock869
BBa_B0011LuxICDABEG (+/-)46Reshma Shetty78In stock1118
BBa_B0025double terminator (B0015), reversed129Caitlin Conboy67In stock1119
BBa_J63002ADH1 terminator from S. cerevisiae225Caroline Ajo-Franklin61It's complicated807
BBa_J61048[rnpB-T1] Terminator113John Anderson50In stock530
BBa_K1051006Saccharomycescerevisiae TBY-1 terminator with stopcodon31Xiang LI43It's complicated825