Frequently Used Parts

The iGEM Registry has a vast collection of parts that have been contributed over the course of our 10 year history. We have more than 20,000 parts that iGEM students (collegiate and high school), graduate students, postdocs, and PIs have contributed to the collection. Some of our parts have been used many, many times. In general, these are the older parts in our collection that have served as the foundation of many projects.

The job of every iGEMer and contributor to the Registry is to make parts users will want to use in coming years. With over 20,000 parts already in our collection, this is not an easy feat. You will need to create great parts for them to become among our most used.

We have many ribosome binding sites in our collection. You may want a strong RBS or one for your organism of choice. However, if you're not sure, you might want one that many other people have used, and will just work in . At the time of writing, our most frequently used part is BBa_B0034. This ribosome binding site, first described by Michael Elowitz in 1999 has been used over 8000 times in the history of the Registry. You can find the number of uses for each part in the part quick reference box at the top of each part page.

The purpose of this page is to showcase the most highly-used parts in our collection so you may see how iGEMers on different teams approach making great parts.

Top 10 Most Used Parts on the Registry

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0034RBS (Elowitz 1999) -- defines RBS efficiency12Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 4940In stock20746
BBa_B0015double terminator (B0010-B0012)129Reshma Shetty4306In stock11115
BBa_R0040TetR repressible promoter54 June Rhee, Connie Tao, Ty Thomson, Louis Waldman1051In stock19575
BBa_B0010T1 from E. coli rrnB80Randy Rettberg956In stock10156
BBa_B0012TE from coliphageT741Reshma Shetty913In stock4154
BBa_B0030RBS.1 (strong) -- modified from R. Weiss15Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 905In stock11741
BBa_B0032RBS.3 (medium) -- derivative of BBa_003013Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 859In stock10737
BBa_E0040 green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212720jcbraff818In stock22388
BBa_R0010promoter (lacI regulated)200 780In stock26325
BBa_J23100constitutive promoter family member35John Anderson657In stock45510

Top 10 Most Used Coding Region Parts

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_E0040 green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212720jcbraff818In stock22388
BBa_E1010 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)706Drew Endy501In stock29881
BBa_C0040tetracycline repressor from transposon Tn10 (+LVA)685June Rhee, Connie Tao, Ty Thomson, Louis Waldman.305In stock12575
BBa_C0051cI repressor from E. coli phage lambda (+LVA) 775Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross277In stock7093
BBa_C0012lacI repressor from E. coli (+LVA)1153Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton257In stock16456
BBa_C0062luxR repressor/activator, (no LVA?)781Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr236In stock33812
BBa_C0061autoinducer synthetase for AHL643Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr209In stock11645
BBa_E0030enhanced yellow fluorescent protein derived from A. victoria GFP723Caitlin Conboy and Jennifer Braff131In stock8152
BBa_E0020engineered cyan fluorescent protein derived from A. victoria GFP 723Caitlin Conboy and Jennifer Braff87In stock7381
BBa_K592009amilCP, blue chromoprotein669Lei Sun80In stock33534

Top 10 Most Used Promoters

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_R0040TetR repressible promoter54 June Rhee, Connie Tao, Ty Thomson, Louis Waldman1051In stock19575
BBa_R0010promoter (lacI regulated)200 780In stock26325
BBa_J23100constitutive promoter family member35John Anderson657In stock45510
BBa_R0011Promoter (lacI regulated, lambda pL hybrid)55Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton584In stock15961
BBa_I0500Inducible pBad/araC promoter1210Sri Kosuri429In stock41577
BBa_R0062Promoter (luxR & HSL regulated -- lux pR)55Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr362In stock61847
BBa_J23119constitutive promoter family member35John Anderson360In stock18080
BBa_J23101constitutive promoter family member35John Anderson291In stock40168
BBa_R0051promoter (lambda cI regulated)49Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross275In stock9224
BBa_J23106constitutive promoter family member35John Anderson224In stock34926

Top 10 Most Used Ribosome Binding Sites

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0034RBS (Elowitz 1999) -- defines RBS efficiency12Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 4940In stock20746
BBa_B0030RBS.1 (strong) -- modified from R. Weiss15Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 905In stock11741
BBa_B0032RBS.3 (medium) -- derivative of BBa_003013Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 859In stock10737
BBa_B0031RBS.2 (weak) -- derivative of BBa_003014Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.215In stock7893
BBa_J61100Ribosome Binding Site Family Member12John Anderson145In stock6179
BBa_Z0262Medium strength T7.2 RBS20Sriram Kosuri123Not in stock3181
BBa_J61101Ribosome Binding Site Family Member12John Anderson104In stock6164
BBa_Z0261Strong T7.2 RBS20Sriram Kosuri98Not in stock3179
BBa_K2066527B0034 with scar TACTAG18Joseph L Maniaci72Not in stock3316
BBa_J176012Kozak18Brady Laughlin56In stock3088

Top 10 Most Used Terminators

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NameDescriptionLengthCreated byUsesStatusDoc
BBa_B0015double terminator (B0010-B0012)129Reshma Shetty4306In stock11115
BBa_B0010T1 from E. coli rrnB80Randy Rettberg956In stock10156
BBa_B0012TE from coliphageT741Reshma Shetty913In stock4154
BBa_B0014double terminator (B0012-B0011)95Reshma Shetty357In stock4662
BBa_B1006Terminator (artificial, large, %T~>90)39Haiyao Huang207In stock1859
BBa_B0025double terminator (B0015), reversed129Caitlin Conboy99In stock4033
BBa_B0011LuxICDABEG (+/-)46Reshma Shetty88In stock4374
BBa_K731721T7 terminator48Giacomo Giacomelli, Anna Depetris75In stock8059
BBa_J63002ADH1 terminator from S. cerevisiae225Caroline Ajo-Franklin66It's complicated3321
BBa_K2486006ECK120029600 - Escherichia coli K-12 terminator90Cauă Westmann and Gabriel Lencioni Lovate60Not in stock2080