Collections/Probiotics

This collection is a user contributed collection, and is not under curation by iGEM HQ/Registry.


This collection was created by iGEM Unesp Brazil 2018 Team

The microbiota plays an important role in several body functions and is correlated to various diseases and dysfunctions. For that reason, probiotics are being engineered to sense and control the production and delivery of therapeutic molecules, along with biocontainment modules to provide the biosafety needed for them to be used as living therapeutics.

To help future teams and scientists interested in engineer probiotics, our team created this collection with parts that have been used and created in the last years by iGEM teams to engineer probiotics using the most common probiotic chassis: Escherichia coli and Escherichia coli Nissle, Lactococcus lactis, Bacillus subtillis and Lactobacillus species.

The collection is organized into 4 categories: sense and control; production and delivery; memory and biocontainment, and according to the chassis in which that part was used or built. In that way, we hope to help people find the necessary modules and parts to build robust living therapeutics.

If your iGEM team is working or has worked with engineered probiotics, please feel free to help this collection grow by labeling your parts in these categories.

Sense and control

Escherichia coli


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_R0082Promoter (OmpR, positive)108Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)27930111RegulatoryIn stock
BBa_K118011PcstA (glucose-repressible promoter)131Andrew Hall1230617RegulatoryIn stock
BBa_K216005PyeaR promoter, responsive to nitrate, nitrite and nitric oxide100Edinburgh iGEM 20092871725RegulatoryIn stock
BBa_K318512gadA (pH promoter and RBS)264Sarah R. Sandock41032RegulatoryIn stock
BBa_K554001flhDC promoter85UNICAMP EMSE Brazil team53345RegulatoryIn stock
BBa_K239005NarK promoter, Fnr activated under anaerobic conditions 139Axel Nystrom38204RegulatoryIt's complicated
BBa_K223044SoxR - SoxS Promoter System776Suzanne Bartram3072 RegulatoryIt's complicated
BBa_K234095Gal 1/10 divergent promoter686Daniel Jedrysiak32682RegulatoryIt's complicated
BBa_K875001T5 Cumate Operator85Federico Colombo, Elisa Clagnan34354RegulatoryIt's complicated
BBa_K1202116pLsr Complete System (Receiver)3450safa tapan2662 CompositeIt's complicated
BBa_K1980004pCusC promoter98Sam Garforth82891RegulatoryIt's complicated
BBa_K1980005pCopA sfGFP810Sam Garforth8288 ReporterIt's complicated
BBa_K1980008pCopA CueR sfGFP/ feedback pCopA sfGFP1252Sam Garforth9203 ReporterIt's complicated
BBa_K2447011Temperature sensitive promoter pTlpA52Chee Wai Kit (david)159211RegulatoryIt's complicated
BBa_K2447013Temperature-sensitive repressible system1409Chee Wai Kit (david)56863RegulatoryIt's complicated
BBa_K223041SoxS Promoter105Suzanne Bartram17544RegulatoryNot in stock
BBa_K2118000atoC Promoter115Laura Ros-Freixedes13411RegulatoryNot in stock
BBa_K2447014IPTG-inducible temperature-sensitive system with GFP reporter3516Chee Wai Kit (david)4172 MeasurementNot in stock
BBa_K2447015Phosphate-dependent, temperature-sensitive cascaded system with GFP reporter 2792Chee Wai Kit (david)2939 MeasurementNot in stock


Lactobacillus spp.


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K1725000PhlF repressible promoter48Mhairi Davidson63586RegulatoryIn stock
BBa_K128006L.bulgaricus LacS Promoter197Derek Ju1680 RegulatoryIt's complicated
BBa_K559011Pgad - Intracellular Chloride-Sensing Cassette1251Jacky, Fong Chuen, Loo113788RegulatoryIt's complicated
BBa_K1130003L. plantarum RBS33Alicia Gabriela Quiroz Rocha 1329 RBSIt's complicated
BBa_K2230012Pcar-wRBS-PhlF-T-Pr-sRBS-GFP/pSB1C31577Yi-Lun Huang45192DeviceIt's complicated
BBa_K2253000Constitutive P8 promoter and RBS composite169Andrew Ly5111 CompositeIt's complicated
BBa_K2253001Constitutive P32 promoter and RBS composite153Andrew Ly5120 CompositeIt's complicated
BBa_K861170PI ,Glucose-activated promoter36Kuanwei Sheng20888RegulatoryNot in stock


Lactococcus lactis


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K1033219Promoter CP160Stephanie Herman, Alona Nyberg4184 RegulatoryIn stock
BBa_K1033220Promoter CP860Stephanie Herman, Alona Nyberg5373 RegulatoryIn stock
BBa_K1033221Promoter CP1159Stephanie Herman, Alona Nyberg54063RegulatoryIn stock
BBa_K1033222Promoter CP2959Stephanie Herman, Alona Nyberg36294RegulatoryIn stock
BBa_K1033223Promoter CP3060Stephanie Herman, Alona Nyberg3630 RegulatoryIn stock
BBa_K1033224Promoter CP4160Stephanie Herman, Alona Nyberg3630 RegulatoryIn stock
BBa_K1033225Promoter CP4459Stephanie Herman, Alona Nyberg67723RegulatoryIn stock
BBa_K2270005gal promoter from Lactococcus lactis218Nathan Vinicius Ribeiro, Danielle Biscaro Pedrolli28512RegulatoryIt's complicated
BBa_K2270008galP-sRNA-rrnb(Bs)327Nathan Vinicius Ribeiro30501CompositeIt's complicated
BBa_K2270006regulatory small RNA for L. lactis109Nathan Vinicius Ribeiro, Danielle Biscaro Pedrolli24001RNAIt's complicated
BBa_K2253000Constitutive P8 promoter and RBS composite169Andrew Ly5111 CompositeIt's complicated
BBa_K2253001Constitutive P32 promoter and RBS composite153Andrew Ly5120 CompositeIt's complicated
BBa_K2660002gal promoter driving the expression of TetR1066Nathan Vinicius Ribeiro15731RegulatoryIt's complicated


Bacillus subtilis


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K2660002gal promoter driving the expression of TetR1066Nathan Vinicius Ribeiro15731RegulatoryIt's complicated
BBa_K2660005T7 RNA Polymerase regulated by TetR2872Danielle Biscaro Pedrolli, Nathan Vinicius Ribeiro16201GeneratorIt's complicated
BBa_K2660010glucose-responsive regulatory circuit5044Danielle Biscaro Pedrolli, Nathan Vinicius Ribeiro2868 GeneratorNot in stock

Production and delivery

Escherichia coli


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K569017CheY418David Golynskiy and Tyler Guinn17742CodingIn stock
BBa_K912000Citrobacter braakii phytase 1302Grant Nicholas24184CodingIn stock
BBa_K899001Fructan fructan fructosyltransferase (FFT)1848Andreas Kjr26694CodingIn stock
BBa_K1033000Tyrosine ammonia-lyase (TAL) with RBS1596Karl Holdar, Hampus Elofsson, Emil Marklund, Kristoffer Lundmark, Marcus Hong, Lovisa Pettersson, Ken Braech-Andersen, Theodor Lwe103202CodingIn stock
BBa_K1692004codon optimized PAL with T7 promoter and Flag Tag1858Daniel Kunin8055 CodingIn stock
BBa_K569007CheZ mutant664David Golynskiy & Tyler Guinn15661CodingIt's complicated
BBa_K899000Sucrose sucrose fructosyltransferase (SST)1893Andreas Kjr27244CodingIt's complicated
BBa_K875004T5LacO-LPP-OmpA-scFv (scFv 54.6 antinorovirus)-6HIS-TT 1516Marija Drikic, Giorgio Gargari36395CompositeIt's complicated
BBa_K875020β-Glucosidase1653Federico Colombo, Elisa Clagnan, Marija Drikic, Giorgio Gargari, Giulia Corso, Sara Samari2714 CompositeIt's complicated
BBa_K875005T5LacO-LPP-OmpA-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT 1906Marija Drikic, Giorgio Gargari3622 CompositeIt's complicated
BBa_K875007T5 LacO-PelB-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT 1529Marija Drikic, Giorgio Gargari3366 CompositeIt's complicated
BBa_K875006T5LacO-PelB-scFv (scFv 54.6 antinorovirus)-6HIS-TT 1121Marija Drikic, Giorgio Gargari3187 CompositeIt's complicated
BBa_K1033002stilbene synthase (STS) with RBS1200Lovisa Pettersson82691CodingIt's complicated
BBa_K10330014-coumarate ligase (4CL) with RBS1708Karl Holdar18302CodingIt's complicated
BBa_K1205000Carnitine Dehydrogenase Protein2431Blake Wilhelmsen3057 CodingIt's complicated
BBa_K1190003Yeb-F GMCSF789Khateeb H. Hussain1284 CodingIt's complicated
BBa_K1033115Histidine Patch Thioredoxin 354Sabri Jamal1665 CodingIt's complicated
BBa_K1639006PotB59-pomA1733Mustafa Yılmaz6508 CodingIt's complicated
BBa_K1639007DAMP-Pexiganan694Mustafa Yılmaz7671 Translational_UnitIt's complicated
BBa_K1639008Tev Protease, single S219V mutation in the internal cleavage site750Mustafa Yılmaz72811CodingIt's complicated
BBa_K1954001Lycopene cassette under the control of NarK, an oxidative stress inducible promoter3532Abbie Rogan46481 CompositeIt's complicated
BBa_K1980000TAT Copper Storage Protein 1 474Sam Garforth8074 CodingIt's complicated
BBa_K1980002MymT183Sam Garforth87003CodingIt's complicated
BBa_K1983013Phenylalanine-specific permease (PheP) from Escherichia coli 1416Vykintas Jaunikis42965CodingIt's complicated
BBa_K2118001patA gene1400Laura Ros-Freixedes16901CodingIt's complicated
BBa_K2118002FMS1 gene1547Laura Ros-Freixedes15751CodingIt's complicated
BBa_K2235011Sialidase enzyme coding composite N terminally attached to secretion system type 1 6915Shivashree Dhanaraj and Shanlin Tong4638 CompositeIt's complicated
BBa_K2230022STM1128/pSB1C31497Yi-Lun Huang & Pei-Hong Chen48031CodingIt's complicated
BBa_K2660001T7 promoter driving 6-his tagged GFP with penetratin981Danielle Biscaro Pedrolli, Paulo Jose Correa Freire3652 ReporterIt's complicated
BBa_K223042Blh Gene: Cleaves beta-carotene into retinal869Suzanne Bartram11734GeneratorNot in stock
BBa_K223043RALDH II gene: Converts retinal into retinoic acid1579Suzanne Bartram11803GeneratorNot in stock
BBa_K1514005TOR-A TAT signaling domain for bacteria159Carlos Goncalves1580 Protein_DomainNot in stock
BBa_K2235005Sialidase enzyme1548Shanlin Tong, Shivashree Dhanaraj, Sina Amoor Pour and Gilai Nachmann27553CodingNot in stock
BBa_K2235008Endo beta galactosidase1293Shanlin Tong, Shivashree Dhanaraj, Sina Amoor Pour and Gilai Nachmann21641CodingNot in stock
BBa_K2660000Penetratin, cell-penetrating peptide51Danielle Biscaro Pedrolli, Paulo Jose Correa Freire21721CodingNot in stock


Lactobacillus spp.


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K1033000Tyrosine ammonia-lyase (TAL) with RBS1596Karl Holdar, Hampus Elofsson, Emil Marklund, Kristoffer Lundmark, Marcus Hong, Lovisa Pettersson, Ken Braech-Andersen, Theodor Lwe103202CodingIn stock
BBa_K128003p1025101Sara Mouradian2479 CodingIt's complicated
BBa_K128004signal sequence from Lactobacillus bulgaricus; secretes protein143Sara Mouradian2336 TagIt's complicated


Lactococcus lactis


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K2230022STM1128/pSB1C31497Yi-Lun Huang & Pei-Hong Chen48031CodingIt's complicated
BBa_K2660011T7 promoter driving mCherry production755Nathan Vinicius Ribeiro13141ReporterNot in stock
BBa_K2660003mCherry codon optimized for Lactococcus lactis724Nathan Vinicius Ribeiro19852CodingNot in stock


Bacillus subtilis


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K2660001T7 promoter driving 6-his tagged GFP with penetratin981Danielle Biscaro Pedrolli, Paulo Jose Correa Freire3652 ReporterIt's complicated
BBa_K2660004Pveg constitutively driving expression of mCherry829Danielle Biscaro Pedrolli, Nathan Vinicius Ribeiro1476 ReporterIt's complicated
BBa_K2660000Penetratin, cell-penetrating peptide51Danielle Biscaro Pedrolli, Paulo Jose Correa Freire21721CodingNot in stock
BBa_K2660011T7 promoter driving mCherry production755Nathan Vinicius Ribeiro13141ReporterNot in stock
BBa_K2660003mCherry codon optimized for Lactococcus lactis724Nathan Vinicius Ribeiro19852CodingNot in stock

Biocontainment

Escherichia coli


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K112000T4 holin, complete CDS, berkeley standard657Bing Xia40056CodingIn stock
BBa_K875009LL 37 - Cathelicidin123Giulia Corso, Sara Samari68094CodingIn stock
BBa_K131010AHL Inducible Colicin E2 with GFP4144Kevin McLeod3309 GeneratorIt's complicated
BBa_K314200Toxin Tse2477Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger42463CodingIt's complicated
BBa_K875008IPTG inducible Tse2 toxin 722Giulia Corso, Sara Samari21872CodingIt's complicated
BBa_K1976048DNase domain of Colicin E2401Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork 35556CodingIt's complicated
BBa_K150007ColicinE1 operon2092Pascal Kraemer17521CompositeNot in stock
BBa_K150010ColicinE9 operon2040Pascal Kraemer17361CompositeNot in stock
BBa_K150012colicinE9 operon (short)1024Pascal Kraemer16311CompositeNot in stock
BBa_K912030pH Inducible Lysis System(w)4515Grant Nicholas1735 CompositeNot in stock
BBa_K912031pH Inducible Lysis System(m)4515Grant Nicholas1735 CompositeNot in stock
BBa_K2447016Phosphate-dependent, temperature-sensitive cascaded system with IM2 expression2333Chee Wai Kit (david)2677 CompositeNot in stock


Lactobacillus spp.


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K117000Lysis gene (promotes lysis in colicin-producing bacteria strain)144Nguyen Xuan Hung897317CodingIn stock
BBa_K1159105Mature Nuclease NucA from Staphylococcus aureus (Thermonuclease) in RFC[25]447Dong-Jiunn Jeffery TRUONG14892CodingIn stock


Lactococcus lactis


There are no parts for this table


Bacillus subtilis


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K2660009pMag450Danielle Biscaro Pedrolli, Victor Nunes de Jesus2272 CodingIt's complicated
BBa_K2660006N-Cas93459Victor Nunes de Jesus, Danielle Biscaro Pedrolli2362 CodingIt's complicated
BBa_K2660007C-Cas9648Danielle Biscaro Pedrolli,Victor Nunes de Jesus2356 CodingIt's complicated
BBa_K2660008nMag450Danielle Biscaro Pedrolli,Victor Nunes de Jesus2257 CodingIt's complicated

Memory

Escherichia coli


There are no parts for this table

Lactobacillus spp.


There are no parts for this table

Lactococcus lactis


There are no parts for this table

Bacillus subtilis


There are no parts for this table