Collections/Cellulose

This collection is under curation by iGEM HQ. New parts and information are currently being added to this page.

Bacterial cellulose has proven to be a useful and flexible biomaterial for a variety of applications. Several iGEM teams have worked with cellulose as a biomaterial, both in production and degradation.


Previous iGEM Team Projects

The following table contains iGEM teams that have worked with cellulose to some degree, along with links to their project wiki and Registry parts. This is not an exhaustive list.

Team Year Parts Project Title
Aalto-Helsinki 2015 Parts Fuel for the Future: E. coli producing renewable propane from cellulose
Berlin 2015 Parts Enzymatic Flagellulose
HAFS-Korea 2015 Parts Engineering an E.coli that transforms cellulose to alcohol by using gene from trichoderma reesei
UCSC 2015 Parts Engineering the Future of Biofuel
Imperial 2014 Parts Manufacture of Bacterial Cellulose Biomaterials: Towards Tailor-Made Biofilters
Hannover 2014 Parts Plant against – Removing heavy metals from nature
StanfordBrownSpelman 2014 Parts Towards a Biosynthetic Unmanned Aerial Vehicle
Biwako Nagahama 2013 Parts AgRePaper&E.coli-ink
Penn State 2013 Parts Plants as Plants: Natural Factories Producing Fuel, Plastic, Flavoring, and More
WHU-China 2012 Parts E. coslim: Synthetic Probiotics Help Defy Obesity
HUST-China 2012 Parts Synthetic Biofactory: Lignocellulose Decomposer and Microbial Fuel Cell
Missouri_Miners 2012 Parts Adjustable Multi-Enzyme to Cell Surface Anchoring Protein
Bielefeld-Germany 2012 Parts TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE
Tec-Monterrey 2011 Parts E. Coli's Sweet Deal
Tokyo_Metropolitan 2010 Parts Life Design: Fine Clothing, Color Housing and Delicious Food by using E. coli


Highlighted Projects

2014 Imperial Team

The iGEM 2014 Imperial Team created a bacterial cellulose filter for their Aqualose project.

"The porosity of bacterial cellulose and our synthetic attachment of contaminant-specific binding and catabolic proteins make for a flexible, modular water filter. Our manufactured biomaterial would augment water recycling and reclamation on local and industrial scales, helping to alleviate water stress." - iGEM 2014 Imperial Team

Through their project, they created a set of well-documented cellulose binding domains, paired with reporter genes (GFP) and metal binding domains.


2014 StanfordBrownSpelman Team

The iGEM 2014 StanfordBrownSpelman Team used cellulose as a biomaterial in their UAV.


Cellulose Synthesis


More...
NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K861100BcsA,cellulose synthetase, catalytic subunit2619Xian Xia13391CodingIn stock
BBa_K861110BcsB,regulator of cellulose synthetase2340Xian Xia12421CodingIn stock
BBa_K336020bacterial cellulose synthase B2415Naoto Shimada 957 CodingIt's complicated
BBa_K336021bacterial cellulose synthase D471Naoto Shimada 950 CodingIt's complicated
BBa_K861120BcsZ, endo-1,4-D-glucanase1107Xian Xia12721CodingIt's complicated
BBa_K1043005Cellulose Synthase 3078Casey Hall828 OtherIt's complicated
BBa_K1043006Cellulose Synthase 82955Casey Hall835 OtherIt's complicated
BBa_K336052bacterial cellulose synthase C3978Naoto Shimada 960 CodingNot in stock
BBa_K336051bacterial cellulose synthase A2238Naoto Shimada 960 CodingNot in stock
BBa_K861130BcsC,cellulose synthetase subunit3474Xian Xia11181CodingNot in stock
BBa_K1044004CelB protein gene CelB from Agrobacterium tumefaciens C58.2484Koki Tsutsumi825 CodingNot in stock
BBa_K1044003Cellulose synthase gene CelA from Agrobacterium tumefaciens C58.2190Koki Tsutsumi867 CodingNot in stock
BBa_K1044005Cellulose synthased operon (CelA,B,C) from Agrobacterium tumefaciens C58.5722Koki Tsutsumi,Eshin Mitsui754 CompositeNot in stock


Cellulose Binding


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NameDescriptionLengthCreated byDocumentationUsesTypeStatus
BBa_K863101Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)345Moritz Müller2670 Protein_DomainIn stock
BBa_K863111Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)318Moritz Müller2616 Protein_DomainIn stock
BBa_K1478001Cellulose binding domain309Fabian Frömling  Protein_DomainIn stock
BBa_K1321344NiBP fused to CBDcex in RFC 25509Chris N Micklem 1CodingIn stock
BBa_K1321346CBDclos fused to sfGFP in RFC 251017Chris N Micklem 1CodingIn stock
BBa_K1321345NiBP fused to dCBD with linker in RFC 25548Chris N Micklem 1CodingIn stock
BBa_K1321352NiBP fused to CBDcex driven by T7567Chris N Micklem  CodingIn stock
BBa_K1321340Double CBD (dCBD) with N-terminal linker366Chris N Micklem 21CodingIn stock
BBa_K1321339CBDcenA+Linker, RFC 25 standard408Chris N Micklem 13CodingIn stock
BBa_K1321090Phytochelatin (PC) EC20 + Linker-dCBD495Xenia Spencer-Milnes  CodingIn stock
BBa_K1321092Phytochelatin (PC) EC20 fused to CBDcex456Xenia Spencer-Milnes  CodingIn stock
BBa_K1321094CBDclos fused to Phytochelatin (PC) EC20429Xenia Spencer-Milnes  CodingIn stock
BBa_K1321343NiBP fused to CBDclos in RFC 25482Chris N Micklem 1CodingIn stock
BBa_K1321342sfGFP fused to CBDcex in RFC 251044Chris N Micklem 3CodingIn stock
BBa_K1321348sfGFP fused to dCBD with linker in RFC 251083Chris N Micklem 1CodingIn stock
BBa_K1321341sfGFP fused to CBDclos in RFC 251017Chris N Micklem 2CodingIn stock
BBa_K1321349CBDcex fused to sfGFP in RFC 251044Chris N Micklem 1CodingIn stock
BBa_K1321351CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)590Chris N Micklem 1CodingIn stock
BBa_K1321353NiBP fused to CBDclos driven by T7540Chris N Micklem  CodingIn stock
BBa_K1321356sfGFP fused to CBDclos driven by LacI1252Chris N Micklem 1CompositeIn stock
BBa_K1321357sfGFP fused to CBDcex driven by LacI1279Chris N Micklem 1CompositeIn stock
BBa_K1499004Cellulose binding domains with streptavidin domain generator1052Raman Nelakanti  GeneratorIn stock
BBa_K1321100Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter553Xenia Spencer-Milnes  CodingIn stock
BBa_K1321101Phytochelatin (PC) EC20 fused to CBDclos with T7 promoter487Xenia Spencer-Milnes  CodingIn stock
BBa_K1321104CBDclos fused to Phytochelatin (PC) EC20 with T7 promoter487Xenia Spencer-Milnes  CodingIn stock
BBa_K1321105CBDcex fused to Phytochelatin (PC) EC20 with T7 promoter514Xenia Spencer-Milnes  CodingIn stock
BBa_K1321110Phytochelatin (PC) EC20 fused to linker-dCBD with LacI 730Xenia Spencer-Milnes  CodingIn stock
BBa_K1321111Phytochelatin (PC) EC20 fused with CBDclos with LacI promoter664Xenia Spencer-Milnes  CodingIn stock
BBa_K1321112Phytochelatin (PC) EC20 fused to CBDcex with LacI promoter691Xenia Spencer-Milnes  CodingIn stock
BBa_K1321114CBDclos fused to phytochelatin (PC) EC20 with LacI promoter664Xenia Spencer-Milnes  CodingIn stock
BBa_K1321115CBDcex fused to phytochelatin (PC) EC20 with LacI promoter691Xenia Spencer-Milnes  CodingIn stock
BBa_K1321120SmtA metallothionein fused to linker-dCBD537Gabriella Santosa  CodingIn stock
BBa_K1321366CBDcex fused to sfGFP with T7 promoter1102Gabriella Santosa  CompositeIn stock
BBa_K1321121Smt metallothionein fused to CBDclos471Gabriella Santosa  GeneratorIn stock
BBa_K1321122Smt fused to CBDcex498Gabriella Santosa  CodingIn stock
BBa_K1321151metallothionein Fmt fused to CBDclos504Gabriella Santosa  CodingIn stock
BBa_K1321152metallothionein Fmt fused to CBDcex531Gabriella Santosa  CodingIn stock
BBa_K1321154CBDclos fused to metallothionein Fmt504Gabriella Santosa  CodingIn stock
BBa_K1321091Phytochelatin (PC) EC20 fused to CBDclos447Xenia Spencer-Milnes  CodingIt's complicated
BBa_K1321095CBDcex fused to Phytochelatin (PC) EC20472Xenia Spencer-Milnes  CodingIt's complicated
BBa_K1321097CBDcenA-linker fused to Phytochelatin (PC) EC20511Xenia Spencer-Milnes  CodingIt's complicated
BBa_K1979001CBD (cbh2)147MA Xinyi -1Coding 
BBa_K1933103constitutive expression of CBDclos fused to BclA with 6xHis tag444Tomoki Uchino  -1Composite 
BBa_K1933102constitutive expression of CBDcex fused to BclA with 6xHis tag471Tomoki Uchino  -1Composite 
BBa_K1933101constitutive expression of CBDclos fused to INPNC with 6xHis tag1335Tomoki Uchino  -1Composite 
BBa_K1933100constitutive expression of CBDcex fused to INPNC with 6xHis tag1362Tomoki Uchino  -1Composite 
BBa_K863102Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)373Moritz Müller2869 CompositeNot in stock
BBa_K863103Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP1111Moritz Müller3576 ReporterNot in stock
BBa_K863110Cellulose binding Domain from Clostridium cellulovorans cellulose binding protein312Moritz Müller2619 Protein_DomainNot in stock
BBa_K863112Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)337Moritz Müller2806 CompositeNot in stock
BBa_K863113Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP1075Moritz Müller3478 ReporterNot in stock
BBa_K863104Cellulose binding Domain from C. Fimi with const. Promotor and GFP1144Moritz Müller3602 ReporterNot in stock
BBa_K863114CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP1117Moritz Müller3566 ReporterNot in stock
BBa_K1321093Phytochelatin (PC) EC20 + Linker-CBDcipA-Linker840Gabriella Santosa 2CodingNot in stock
BBa_K1321003Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25327Xenia Spencer-Milnes 23CodingNot in stock
BBa_K1321002Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25300Xenia Spencer-Milnes 24CodingNot in stock
BBa_K1321014CBDCipA with N and C-terminal linker711Xenia Spencer-Milnes 7CodingNot in stock
BBa_K1321096Linker-CBDcipA-Linker + Phytochelatin (PC) EC20840Xenia Spencer-Milnes 2CodingNot in stock


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